Mutations frequently found in patients whose leukemia are resistant to standard chemotherapy, with a poor prognosis with a poor prognosis In the experimental model of the crew employed the shRNAs or or activated In In leukemic mice by supplementing their drinking water with the drug doxycycline – ‘Induction of shRNA, which shut down a necessary gene for the survival of leukemia cells may lead to complete remission of the disease,’explains Johannes Zuber, a former CSHL postdoctoral fellow in the lab Lowe, who has helped this approach and who founded a short he has his own group at the Research Institute of Molecular Pathology in Vienna.
‘Cancer is clearly a genetic disease, but we also appreciate that epigenetic changes, how genes are expressed, contribute to the uncontrolled growth of cancer cells,’said Vakoc. Cancer cells use these altered epigenetic landscape take their cell growth programs.. Screened in a single experiment, in which she focused more than 1000 shRNAs targeting 243 known epigenetic regulators of chromatin, the team of scientists in a target. BRD4 suppress BRD4 with a shRNA resulted in a dramatic cell cycle arrest and death of leukemia cells and a significant delay in leukemia progression and significantly the survival of leukemia cells extended animals.
The target protein in the RNAi screen identified described in the current study BRD4 – which contains a distinct domain or region known as the bromodomain – is a member of the BET family of proteins the gene expression the gene expression. Continue reading