If the same restriction site sequence of the entire gene occurred, would be the specific restriction enzyme that recognizes this site make several cuts on Richardson. ‘That would make it impossible for the precise cutting and pasting needed to make and use artificial genes to do,’she said.
To enable the use of artificial genes we need millions of millions of copies of them for experiments using a process called polymerase chain reaction, said Boeke. By restriction sites at specific points along the gene, we can do it in bite-sized pieces School of Medicine,ed millions of times truncated. Thus, the ability to cut and paste genes back together is critical for the design of genes to the correct specifications, they replicate quickly and putting them into vectors, genetically engineer cells. .
The other authors of the paper Sarah J. Wheelan and Robert M. Yarrington at Johns Hopkins University School of Medicine, High Throughput Biology Center are.. But a successfully designed gene would not benefit researchers if is only one copy it. Continue reading